ALEXANDRIA, Va., Nov. 6 -- United States Patent no. 12,460,215, issued on Nov. 4, was assigned to WILLIAM MARSH RICE UNIVERSITY (Houston).
"Bacillus expression system" was invented by Jeffrey J. Tabor (Houston) and Sebastian M. Castillo-Hair (Houston).
According to the abstract* released by the U.S. Patent & Trademark Office: "An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into the B. subtilis genome. In the absence of IPTG, Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF-here superfolder green fluorescent protein (sfGFP) reporter protein-is undetectable by flow cytometry...