ALEXANDRIA, Va., June 25 -- United States Patent no. 12,338,454, issued on June 24, was assigned to TOYOTA JIDOSHA K.K. (Toyota, Japan).
"Helper plasmid for transformation, method for producing transformant using the same, and transformation method" was invented by Toru Onishi (Toyota, Japan) and Nobuki Tada (Nisshin, Japan).
According to the abstract* released by the U.S. Patent & Trademark Office: "A stable transformant, in which a gene of interest is incorporated into the genome, is simply and efficiently produced.A method for producing a transformant, comprising a step of introducing into a host, linear genome-introduced nucleic acid fragment(s) comprising a gene of interest and a helper plasmid for transformation having a pair of hom...