US Patent Issued to The Johns Hopkins University on July 15 for "Bottleneck sequencing" (Maryland Inventors)

ALEXANDRIA, Va., July 16 -- United States Patent no. 12,359,250, issued on July 15, was assigned to The Johns Hopkins University (Baltimore).

"Bottleneck sequencing" was invented by Bert Vogelstein (Baltimore), Kenneth Kinzler (Baltimore), Margaret Hoang (Baltimore) and Nickolas Papadopoulos (Towson, Md.).

According to the abstract* released by the U.S. Patent & Trademark Office: "Bottleneck Sequencing System (BotSeqS) is a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately prior to library amplification. BotSeqS can be used to show age and tissue-dependent accumulations of r...