US Patent Issued to Siemens Healthineers on Dec. 2 for "Direct RNA nanopore sequencing with help of a stem-loop reverse polynucleotide" (German Inventor)

ALEXANDRIA, Va., Dec. 2 -- United States Patent no. 12,486,533, issued on Dec. 2, was assigned to Siemens Healthineers AG (Erlangen, Germany).

"Direct RNA nanopore sequencing with help of a stem-loop reverse polynucleotide" was invented by Yiwei Huang (Erlangen, Germany).

According to the abstract* released by the U.S. Patent & Trademark Office: "A method includes (i) providing an RNA polynucleotide; (ii) modifying the RNA polynucleotide by annealing and ligating a polynucleotide comprising a 3' terminal random multimer segment and having a stem-loop form; (iii) optionally performing a reverse transcription of the RNA polynucleotide; (iv) cleaving the stem-loop segment of the annealed polynucleotide to yield a 3' A overhang; (v) connectin...