ALEXANDRIA, Va., Feb. 3 -- United States Patent no. 12,540,317, issued on Feb. 3, was assigned to ProteoNic Biotechnology IP B.V. (Leiden, Netherlands).
"Self-immolative plasmid backbone" was invented by Bart Marinus Engels (Woerden, Netherlands), Raymond Michael Dimphena Verhaert (Breda, Netherlands) and Maurice Wilhelmus Van der Heijden (Waddinxveen, Netherlands).
According to the abstract* released by the U.S. Patent & Trademark Office: "The invention relates to a method for separating a polynucleotide insert from a polynucleotide vector backbone. The backbone has a plurality of cleavage sites distributed such that the backbone is converted into fragments when the sites are cleaved. This allows straightforward separation of the insert ...