ALEXANDRIA, Va., July 16 -- United States Patent no. 12,359,227, issued on July 15, was assigned to Novozymes A/S (Bagsvaerd, Denmark).

"Polypeptides having pullulanase activity suitable for use in liquefaction" was invented by Takashi Nakanishi (Chiba, Japan), Aki Tomiki (Chiba, Japan) and Yuma Kurakata (Chiba, Japan).

According to the abstract* released by the U.S. Patent & Trademark Office: "The present invention relates to a variant pullulanase, having increased thermo-stability and/or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399...