US Patent Issued to National Research Council of Canada on May 13 for "E. coli deficient in Lon and SulA for gene targeting" (Canadian Inventors)

ALEXANDRIA, Va., June 12 -- United States Patent no. 12,297,239, issued on May 13, was assigned to National Research Council of Canada (Ottawa).

"E. coli deficient in Lon and SulA for gene targeting" was invented by Luke Masson (Dollard-des-Ormeaux, Canada) and Melanie Arbour (Ste-Marthe-sur-le-Lac, Canada).

According to the abstract* released by the U.S. Patent & Trademark Office: "An expression system and process for the production of Diphtheria toxin polypeptides or mutated forms thereof, such as the toxoid CRM197 polypeptide, in genetically-modified E. coli with high yield is described. The system and process is based on the uncoupling of biomass growth from recombinant protein induction, i.e. using an inducer of protein production th...