ALEXANDRIA, Va., Sept. 23 -- United States Patent no. 12,421,533, issued on Sept. 23, was assigned to NANJING NUTRABUILDING BIO-TECH Co. LTD. (Nanjing, China).
"Ean B mutants and their uses" was invented by Qilin Liao (Nanjing, China) and Jian Zhang (Nanjing, China).
According to the abstract* released by the U.S. Patent & Trademark Office: "Provided herein includes a method for producing ergothioneine, comprising N(Alpha)-trimethyl histidine and an oxidative sulfurizing enzyme mutant. With the mutant enzyme's help, the conversion rate is higher than 30% with the mutant enzyme amount of 8000/g substrate in 24 hours. Disclosed are a nucleic acid encoding the mutant enzyme, an expression vector comprising the nucleic acid, an expressing hos...