ALEXANDRIA, Va., Oct. 28 -- United States Patent no. 12,454,688, issued on Oct. 28, was assigned to Illumina Inc. (San Diego).
"Library preparation of tagged nucleic acid using tube add-on protocol" was invented by Fiona Kaper (San Diego) and Gordon Cann (Hayward, Calif.).
According to the abstract* released by the U.S. Patent & Trademark Office: "A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate; inactivating the protease to generate an inactivated cell lysate, and applying a transposase and a transposon end composition containing a transferred strand to the inactivated cell lysate under conditions...