ALEXANDRIA, Va., Aug. 26 -- United States Patent no. 12,398,382, issued on Aug. 26, was assigned to IDEMITSU KOSAN Co. LTD. (Chiyoda-ku, Japan).
"Soluble expression of protein using peptide tag" was invented by Kazuyoshi Koike (Sodegaura, Japan), Eiji Takita (Sodegaura, Japan) and Takeshi Matsui (Sodegaura, Japan).
According to the abstract* released by the U.S. Patent & Trademark Office: "By linking a peptide tag having the amino acid sequence X(PY)qPZ to a protein of interest, and then expressing the protein, the protein of interest is efficiently accumulated in a soluble fraction, where P is proline; X is an amino acid sequence composed of 0 to 5 amino acids independently selected from R, G, S, K, T, L, N, Q, and M; Y is an amino acid ...