ALEXANDRIA, Va., July 30 -- United States Patent no. 12,371,679, issued on July 29, was assigned to European Molecular Biology Laboratory (Germany).
"Transfection method using a sleeping beauty transposase variant with increased solubility that transfects a target cell when used in protein form" was invented by Orsolya Barabas (Gaiberg, Germany), Irma Querques (Zurich) and Cecilia Ines Zuliani (Heidelberg, Germany).
According to the abstract* released by the U.S. Patent & Trademark Office: "The present invention relates to improved transposase polypeptides having increased solubility. The enzyme of the invention was developed based on the Sleeping Beauty (SB) transposase. The invention provides further nucleic acids, vectors and recombina...