US Patent Issued on Oct. 7 for "Reaction condition composition for circularizing oligonucleotide probes" (Nebraska Inventors)

ALEXANDRIA, Va., Oct. 8 -- United States Patent no. 12,435,360, issued on Oct. 7.

"Reaction condition composition for circularizing oligonucleotide probes" was invented by Abraham Oommen (Lincoln, Neb.), Heather Piscatelli (Lincoln, Neb.) and Alyssa Hangman (Lincoln, Neb.).

According to the abstract* released by the U.S. Patent & Trademark Office: "Reaction condition compositions for detecting a genomic variation from a small sample amount from 5 nano grams (ng) to 1 microgram (ug) includes DNA ligase, DNA polymerase, at least one COP, a DNA polymerase buffer, NAD+, at least two primers, and deoxynucleotide triphosphates (dNTPs). Detection of the genomic variation utilizes COPs with increased ligation efficiency and RCA with fluorescence ...