US Patent Issued to The Florida International University Board of Trustees on Feb. 25 for "High throughput screening assay to identify DNA topoisomerase inhibitors" (Florida Inventors)

ALEXANDRIA, Va., Feb. 26 -- United States Patent no. 12,234,504, issued on Feb. 25, was assigned to The Florida International University Board of Trustees (Miami).

"High throughput screening assay to identify DNA topoisomerase inhibitors" was invented by Fenfei Leng (Palmetto Bay, Fla.) and Matthew Dias (Miami).

According to the abstract* released by the U.S. Patent & Trademark Office: "The subject invention provides a novel fluorescence-based, T5 exonuclease-amplified DNA cleavage assay for gyrase poisoning inhibitor discovery. According to the assay, multiple gyrase molecules can simultaneously bind to a plasmid DNA molecule to form multiple gyrase-DNA cleavage complexes on the same plasmid. These gyrase-DNA cleavage complexes, greatly ...